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Estrogen Metabolites Level 3-24 Hour Urine (Test Code 1209)

Collection Method

24 Hour Urine

Turn Around Time

10-12 Business Days


Sample Report


The estrogen metabolite assessment detects the urinary products of estrogen metabolism. The metabolites have their own specific biological actions; some have the stimulatory properties of estradiol, whilst others are weak and protective estrogen molecules. An imbalance in estrogen metabolism has been associated with osteoporosis, high blood pressure, lupus and cancer (breast, endometrial, prostate, thyroid, head and neck). As the estrogen metabolites are modifiable by lifestyle and nutritional changes, this test is useful for not only establishing risk but monitoring therapies which can reduce the probability of these disorders.

Estrogen has positive effects on the cardiovascular system, bones, brain, skin as well as reproductive organs. The issues experienced at menopause such as memory loss, osteoporosis and vaginal dryness validates the importance of maintaining optimal estrogen levels. However, if you have too much estrogen in the body, it may increase the risk of hormone-related cancers such as endometrial, prostate and breast cancer.

The view that all estrogens are ‘bad’ and cause cancer is a simplistic one. However, Estradiol (E2) and Estrone (E1) are metabolised into at least 14 different species. These breakdown products and have their own specific biological actions; some have the stimulatory properties of estradiol whilst others are weak and antagonistic to other estrogen molecules. An imbalance of the stimulatory versus weak estrogen metabolites have been observed to be associated with many different health disorders. Whilst high levels of the 16α-hydroxyestrone stimulatory estrogen raise cancer risk, lower levels are associated with osteoporosis. In contrast, if the weak estrogens are high they protect against cancer but inhibit bone growth. Therefore the estrogen metabolites are not inactive excretion products but powerful molecules in their own right and should be monitored.

The estrogen metabolites profile level 3 is the most comprehensive assessment as it looks at a comprehensive scope of estrogen metabolites for the prevention and treatment of estrogen-related symptoms and conditions. This robust urine analysis is ideal for monitoring and assessing the risk of estrogen-related diseases.

Common Conditions:

  • Anxiety
  • Osteoporosis
  • Depression
  • Uterine Fibroids
  • Fatigue
  • Insomnia
  • Poor Memory
  • Headaches/Migraines
  • Loss of libido
  • Immune dysregulation
  • Cancer
  • Mood Disorders
  • Fatigue
  • Cardiovascular disease
  • Menopausal symptoms
  • Joint pain
  • Premenstrual syndrome
  • Muscle pain


FostisT, Adlercruetz  H. (1987).The multi-component analysis of estrogens in urine by ion-exchange chromatography and GC-MS- – 1 Quantitation of  estrogens  after initial  hydrolysis of  conjugates.  J Steroid   Biochem   28:203-213.

24 Hour Urine - Level 1 - Test Code 1207

2-hydroxyestrone (2OHE1), 16α-hydroxyestrone (16αOHE1); 2OHE1:16αOHE1 ratio.

24 Hour Urine - Level 2 - Test Code 1208

2-hydroxyestrone (2OHE1), 4-hydroxyestrone (4OHE1), 16α-hydroxyestrone (16αOHE1); 2OHE1:16αOHE1 ratio.

24 Hour Urine - Level 3 - Test Code 1209

2-hydroxyestrone (2OHE1), 4-hydroxyestrone (4OHE1), 16α-hydroxyestrone (16αOHE1); 2OHE1:16αOHE1 ratio, 2-methoxyestrone (2OMeE1), 4-methoxyestrone (4OMeE1); 2OHE1:2OMeE1 ratio, 4OHE1:4OMeE1 ratio.

Test Method



2-hydroxyestrone (2OHE1)
4-hydroxyestrone (4OHE1)
16α-hydroxyestrone (16αOHE1)
2OHE1:16αOHE1 ratio
2-methoxyestrone (2OMeE1)
4-methoxyestrone (4OMeE1)
2OHE1:2OMeE1 ratio
4OHE1:4OMeE1 ratio

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NUTRIPATH offers more tests than any other integrative medical laboratory. With more than 200 tests available, it is the anti-aging and integrative medical practitioners’ one-stop-shop with assessments that investigate endocrine, gastrointestinal, immunological, genomic, metabolic and nutritional balance.

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